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Lecturer(s)
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Valenta Libor, Mgr. Ph.D.
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Uhlíř Petr, Mgr. Ph.D.
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Soukupová Klára, Mgr.
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Course content
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1. Labor safety 2. History of microscopic techniques 3. Basic optical principles 4. Construction of microscope, preparation of preparations. 5. Working with a stereoscopic microscope. 6. Electron microscopy, preparation of electron microscopy specimens 7. Pipetting, DNA isolation, agar gel preparation and DNA visualization on the gel, preparation of bacterial cultures. 8. Collection, preparation and preservation of plant and animal material 9. Types of staining of microscopic preparations 10. Watering of the tissue into paraffin binders, cutting and preparation of preparations 11. Documentation of biological objects (digital photography, drawing and computer graphics). 12. Basic laboratory and measuring instruments in biology (pH meter, oximeter, fluorimeter, electrophoresis, centrifuge, etc.). 13. Credit test.
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Learning activities and teaching methods
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Laboratory work, Lecture with visual aids
- Practical training (number of hours)
- 13 hours per semester
- Contact hours
- 13 hours per semester
- Preparation for comprehensive test (10-40)
- 25 hours per semester
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| prerequisite |
|---|
| Knowledge |
|---|
| know labor safety in the laboratory |
| be able to distinguish laboratory equipment and aids |
| know ways of collecting, preparing and preserving plant and animal material |
| know the principles of light and electron microscopy |
| to be able to prepare microscopic preparations and be able to work with a stereoscopic microscope |
| know some basic laboratory methods used in biology and be able to document biological objects (classic and digital photography, drawing and computer graphics) |
| Skills |
|---|
| to be able to work with a light microscope and a stereomicroscope |
| on the basis of the assigned task to specify the goals, results and conclusions of their own observation at the gymnasium level |
| to create a simple expert text at a simple level |
| Competences |
|---|
| N/A |
| N/A |
| N/A |
| learning outcomes |
|---|
| Knowledge |
|---|
| know the construction of light and electron microscopy |
| know the basics of collecting and preparing biological material |
| know the principles of pipetting by glass and automatic pipette |
| know basic documentation methods |
| Skills |
|---|
| to able to prepare the microscopical preparates |
| be able to create a simple expert text |
| Competences |
|---|
| N/A |
| N/A |
| teaching methods |
|---|
| Knowledge |
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| Lecture with visual aids |
| Laboratory work |
| Skills |
|---|
| Laboratory work |
| Competences |
|---|
| Lecture with visual aids |
| assessment methods |
|---|
| Knowledge |
|---|
| Test |
| Skills |
|---|
| Skills demonstration during practicum |
| Competences |
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| Skills demonstration during practicum |
| Test |
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Recommended literature
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Sběr, preparace a konzervace rostlinného materiálu : Určeno pro posl. fak. přírodověd.. 2., přeprac. vyd. Praha : SPN, 1989. ISBN 80-7066-034-1.
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Habrová, V. Mikroskopická technika. SPN Praha, 1985.
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Kalina, T., Váňa J. Sinice, řasy, houby, mechorosty a podobné organismy v současné biologii. Praha, 2005.
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Knoz, J., Opravilová, V. Základy mikroskopické techniky. Brno, 1992.
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Lelláková, F. a kol. Zoologická technika. UK Praha, 1985.
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Rosypal, S. Bakteriologie a virologie. Praha, 1994.
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