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Lecturer(s)
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Course content
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1. Eukaryotic and prokaryotic cell - organelles, intercellular connections, chemical composition, cell vital functions 2. Cells - metabolism, movement, communication, transport, growth, reproduction - mitosis, meiosa, etc. 3. Structure of DNA, RNA, proteins, central dogma of molecular biology, genetic code 4. Structure of the prokaryotic and eukaryotic genome 5. Gene families, repetitive sequences, transposable element, organelle genomes 6. Replication, transcription and post-transcriptional modifications 7. Translations and post-translational modifications, regulation of gene expression, methylation and other DNA modifications 8. Cell cycle control, differentiation, apoptosis, mutation, repair, tumor diseases, operons, phage expression, controlled eukaryotic transcription, signal molecules 9. Methods of molecular biology - nucleic acid isolation, centrifugation, electrophoresis, restriction enzymes 10. PCR, cloning, NK labeling, hybridization 11. Sequencing, sequencing of the new generation 12. Chromatography, in vitro translation, FISH, DNA chips 13. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Their Possibilities for the Future
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Learning activities and teaching methods
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Students' self-study, Self-study of literature, Textual studies, Lecture, Lecture with visual aids
- Preparation for comprehensive test (10-40)
- 26 hours per semester
- Preparation for an examination (30-60)
- 26 hours per semester
- Contact hours
- 26 hours per semester
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| prerequisite |
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| Knowledge |
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| know and control chemical nomenclature Know the biochemical nature of the events that take place in living organisms control biology and chemistry at the gymnasium level to know the cell construction at the gymnasium level to know the professional terms of general biology at the gymnasium level |
| Skills |
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| use independent thinking and have the ability to study literature on the basis of the assigned task to specify the goals, results and conclusions of their own observation at the gymnasium level |
| Competences |
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| N/A |
| learning outcomes |
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| Knowledge |
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| To know the structure and composition of viruses, eukaryotic and prokaryotic organisms. |
| Know the forms of genetic information, its transmission and adaptations. |
| Describe replication, transcription and translation. |
| To know and explain the dogma of molecular biology and describe all the processes that are related to it. |
| To know differentiation, apoptosis and other similar events taking place in the body. |
| Know PCR and other molecular biology methods. |
| Skills |
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| Use basic terminology of molecular biology. |
| Suggests to use basic methods of molecular biology. |
| Competences |
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| N/A |
| teaching methods |
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| Knowledge |
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| Lecture |
| Lecture with visual aids |
| Textual studies |
| Self-study of literature |
| Skills |
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| Lecture |
| Competences |
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| Lecture |
| assessment methods |
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| Knowledge |
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| Continuous assessment |
| Written exam |
| Skills |
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| Combined exam |
| Competences |
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| Combined exam |
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Recommended literature
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Alberts, B. a kol. Základy buněčné biologie : úvod do molekulární biologie buňky. Ústí nad Labem: Espero Publishing, 1998. ISBN 80-902906-2-0.
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Alberts B. Molecular Biology of the Cell. New York: Garland Science, 2014.
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Jelínek, Jan. Úvod do biochemie a molekulární biologie (nejen) pro gymnázia. Vydání první. 2021. ISBN 978-80-7402-440-5.
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Kubišta, V. Obecná biologie. Fortuna Praha, 2000.
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Rosypal, S. a kol. Nový přehled biologie. Praha, 2003.
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Rosypal, Stanislav. Úvod do molekulární biologie. 2.,rozš. vyd. Brno : Stanislav Rosypal, 1997.
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Šmarda J. Metody molekulární biologie. Masarykova univerzita, 2010. ISBN 9788021038417.
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Žurovec, J. Molekulární biologie živočichů. Biologická fakulta JCU, 1999.
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